LOCUS       40924_23599       11414 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB659 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11414)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2065..2775
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      2800..3052
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3086..3102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3110..3126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3134..3164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3179..3200)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3236..3488)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3651..4199)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4251..4430)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4936..4960)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5481..6269
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6518..7106
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7292..7432)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7776..7970)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8039..9109)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9541..10167)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggtg tctaagggcg aagagctgat taaggagaac
     2101 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
     2161 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     2221 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc
     2281 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
     2341 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     2401 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
     2461 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac
     2521 cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc
     2581 cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     2641 atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
     2701 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     2761 gggcacaaac ttaattgagc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     2821 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     2881 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2941 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3001 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3061 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3121 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3181 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct
     3241 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     3301 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     3361 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     3421 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     3481 gaacgatcgg ggaaattcga gctggggacg tccccagctc ggtaccccct cctgggcacg
     3541 gtcgtacgcg atccgccgcg gttccacctc accattgcgg gtatgccagt tgagttcgat
     3601 gagttcccgt accagctcca actcgggcga tgccacgcgc actccgttca gatctcggtg
     3661 acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg
     3721 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg
     3781 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc
     3841 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc
     3901 gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg
     3961 cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg
     4021 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc
     4081 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc
     4141 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcata
     4201 tcttattgcc ccccgggatc agatctggat tgagagtgaa tatgagactc taattggata
     4261 ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga
     4321 tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc
     4381 attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc
     4441 aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc
     4501 tccgctcatg atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     4561 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     4621 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     4681 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     4741 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     4801 ttgcgccctg agtgcttgcg gcagcgtgaa gctgatccgt cgagattaat agaaataaat
     4861 tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     4921 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     4981 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     5041 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     5101 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     5161 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     5221 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     5281 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     5341 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     5401 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     5461 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     5521 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     5581 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     5641 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     5701 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     5761 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     5821 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     5881 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     5941 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     6001 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     6061 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     6121 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     6181 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     6241 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     6301 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     6361 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     6421 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
     6481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6661 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7141 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7201 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7261 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     7321 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     7381 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     7441 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7501 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     7561 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     7621 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
     7681 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     7741 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     7801 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     7861 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     7921 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     7981 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     8041 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     8101 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     8161 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     8221 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     8281 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     8341 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
     8401 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
     8461 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
     8521 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
     8581 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
     8641 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
     8701 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
     8761 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
     8821 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
     8881 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
     8941 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
     9001 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
     9061 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
     9121 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg
     9181 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
     9241 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
     9301 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
     9361 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
     9421 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
     9481 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
     9541 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
     9601 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
     9661 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
     9721 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
     9781 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
     9841 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
     9901 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
     9961 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
    10021 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
    10081 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
    10141 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
    10201 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
    10261 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
    10321 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
    10381 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
    10441 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
    10501 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
    10561 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
    10621 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
    10681 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
    10741 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    10801 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    10861 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    10921 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    10981 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    11041 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    11101 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    11161 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    11221 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    11281 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    11341 ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac
    11401 gaacggataa acct
//
